mouse albumin assaymax elisa kit  (Assaypro)

Journal: International Journal of Molecular Sciences

Article Title: Metabolic and Antioxidant Modulation by Artemisia indica Willd. Aqueous Extract in Glucose and Cholesterol Dysregulation

doi: 10.3390/ijms27010297

Figure Lengend Snippet: AAE inhibited polyol pathway activity and glycogen accumulation in HFD/STZ mice. The levels of aldose reductase ( a ), fructose ( b ), methylglyoxal ( c ), and carboxymethyl-lysine ( d ) were detected by a commercially available ELISA kit. ( e ) Pathological changes were evaluated by periodic acid–Schiff (PAS) staining, showing glycogen accumulation and abnormal polysaccharides (400× magnification). ( f ) PAS-positive areas in kidney tissues were quantified. Data were collected per group and are presented as the mean ± SD ( n = 5), derived from five independent biological replicates. # p < 0.05, ## p < 0.01 versus the control group; * p < 0.05, ** p < 0.01 versus the model group. AAE: Artemisia indica Willd. aqueous extract; HFD: high-fat diet; PAS: periodic acid–Schiff; STZ: streptozotocin.

Article Snippet: A normalized analysis was conducted by measuring urinary albumin (Mouse Albumin AssayMax ELISA Kit, Assaypro, Saint Charles, MO, USA, Cat# EMA3201-1) and urinary creatinine (Cayman Creatinine ELISA Kit, Item No. 502330, Michigan, USA), and subsequently, the urinary albumin-to-creatinine ratio (UACR) was calculated [ ].

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Staining, Derivative Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Metabolic and Antioxidant Modulation by Artemisia indica Willd. Aqueous Extract in Glucose and Cholesterol Dysregulation

doi: 10.3390/ijms27010297

Figure Lengend Snippet: AAE enhanced antioxidant defense in HFD/STZ mice. The content of antioxidant enzymes, including glutathione (GSH) ( a ), superoxide dismutase (SOD) ( b ), and catalase ( c ), was detected by ELISA assays. ( d ) Malondialdehyde (MDA). Data were collected per group and are presented as the mean ± SD ( n = 5), derived from five independent biological replicates. # p < 0.05, ## p < 0.01 versus the control group; ** p < 0.01 versus the model group. AAE: Artemisia indica Willd. aqueous extract; HFD: high-fat diet; STZ: streptozotocin.

Article Snippet: A normalized analysis was conducted by measuring urinary albumin (Mouse Albumin AssayMax ELISA Kit, Assaypro, Saint Charles, MO, USA, Cat# EMA3201-1) and urinary creatinine (Cayman Creatinine ELISA Kit, Item No. 502330, Michigan, USA), and subsequently, the urinary albumin-to-creatinine ratio (UACR) was calculated [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Control

Journal: Frontiers in Physiology

Article Title: Proteomic analyses of urinary exosomes identify novel potential biomarkers for early diagnosis of sickle cell nephropathy, a sex-based study

doi: 10.3389/fphys.2024.1300667

Figure Lengend Snippet: Sickle cell disease (SCD) mice have albuminuria when they reach 4 months old. (A) At 2 months old, there was no significant difference in urinary albumin excretion between SCD and C57BL/6 mice ( n = 3, 2 males and 1 female in each strain). (B) By the age of 4 months, SCD mice exhibited a more than threefold increase in urinary albumin excretion compared to their levels at 2 months old (* p < 0.05, n = 5, 2 males and 3 females). It is worth noting that the disparities in urinary albumin levels observed between (A,B) for 2-month-old SCD mice are likely attributable to variations in measurement times and the use of different sets of SCD mice for each measurement. Urinary albumin levels were quantified using an ELISA kit (EMA3201, AssayPro). (C) SCD mice showed significantly elevated transcutaneous glomerular filtration rate (tGFR) compared to C57BL/6 mice (* p < 0.05, t -test, n = 5, 2 males and 3 females). The tGFR was determined by measuring the disappearance of fluorescein isothiocyanate (FITC)-sinistrin from the mouse body. (D) At 4 months old, SCD mice displayed enlarged glomeruli and minor fibrosis (indicated by arrows) compared to non-SCD mice (heterozygotes), as shown in representatives from three independent experiments. H & E, hematoxylin-eosin staining; MTC, Masson’s trichrome staining.

Article Snippet: Mouse urine albumin levels were measured using the Mouse Albumin Assay Max ELISA kit (catalog # EMA3201-1 from AssayPro) according to the manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Filtration, Staining